Device
Part:BBa_K329008
Designed by: Raphael Pantier Group: iGEM10_Paris_Liliane_Bettencourt (2010-10-12)
Double terminator (B0015) - attC - mRFP
This part is composed of a double terminator (BBa_B0015) followed by an attC site and mRFP (with an RBS). When the IntI integrase is expressed, it can specifically excise a region of DNA flanked by attC sites.
Important note : RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 205
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 205
Illegal NotI site found at 1081 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 205
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 205
Illegal AgeI site found at 784
Illegal AgeI site found at 896 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |